Development of Real-Time PCR Assays for the Detection of Lupin Residues in Food Products
Lupin is a legume that is used for human nutrition. Unfortunately lupin is known to trigger allergic reactions and therefore a mandatory declaration on the label of foodstuffs is required (Directive 2007/68/EC). To protect the allergic consumer lupin detection methods are needed. Here we present the development of two real-time polymerase chain reaction (PCR) methods that allow the detection of lupin-specific DNA. In cookies lupin could be detected at a level of only 5 mg per kg food matrix ELISA analyses were performed to compare the detection of lupin DNA with that of lupin protein, and the real-time PCR method targeting d-conglutin was shown to yield results comparable to those obtained by ELISA.
GOMEZ GALAN Antonio Manuel;
BROHEE Marcel;
SCARAVELLI Elena;
VAN HENGEL Adrianus;
CHASSAIGNE Hubert;
2010-02-05
SPRINGER
JRC48133
1438-2377,
https://publications.jrc.ec.europa.eu/repository/handle/JRC48133,
10.1007/s00217-009-1199-5,
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