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dc.contributor.authorDE BAETS Liesbeten_GB
dc.contributor.authorVAN IWAARDEN Pierreen_GB
dc.contributor.authorBREMSER Wolframen_GB
dc.contributor.authorMEEUS Neleen_GB
dc.contributor.authorPHILIPP Wolfgangen_GB
dc.contributor.authorSCHIMMEL Heinzen_GB
dc.date.accessioned2010-02-25T18:19:15Z-
dc.date.available2008-11-05en_GB
dc.date.available2010-02-25T18:19:15Z-
dc.date.created2008-11-05en_GB
dc.date.issued2008en_GB
dc.date.submitted2008-10-21en_GB
dc.identifier.isbn978-92-79-10271-4en_GB
dc.identifier.issn1018-5593en_GB
dc.identifier.otherEUR 23540 ENen_GB
dc.identifier.otherOPOCE LA-NA-23540-EN-Cen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC48342-
dc.description.abstractThis report describes the certification of a reference material (IRMM-351) of Escherichia coli O157. Certified Reference Materials (CRMs) for microbiological analysis are indispensable tools for development and validation of detection methods and for the implementation and support of internal and external quality control in the area of microbiological analysis. Each vial contains one material sphere of E. coli O157. The homogeneity and stability (at 4 °C, -20 °C and -70 °C) of the batch was assessed by monitoring colony forming units (cfu) on nutrient agr (NA) and eterohemolysin agar (EhlyA) of selected vials by colony counting. The material is not stable at 4 °C but no instability was detected when stored at -20 °C for up to 12 months and at -70 °C for up to 54 months. The batch was characterised by six laboratories to determine a certified value of cfu per vial on NA and EhlyA. The certified value is 4 cfu on both agars with an expanded uncertainty of 2 using a coverage factor k = 2, corresponding to a level of confidence of about 95 %. DNA sequence analysis of the coding region for the fliC gene identified the material as E. coli O157.en_GB
dc.description.sponsorshipJRC.D.2-Reference materialsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherOPOCEen_GB
dc.relation.ispartofseriesJRC48342en_GB
dc.titleCertification of a Reference Material with Eschericia Coli O157 (NCTC 12900) at a level of 4 Colony forming Unit per Material Sphere, IRMM-351en_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
dc.identifier.doi10.2787/84342en_GB
JRC Directorate:Health, Consumers and Reference Materials

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