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|Title:||Bioadhesive Nanoareas in Antifouling Matrix for Highly Efficient Affinity Sensors|
|Authors:||MANNELLI Ilaria; VALSESIA Andrea; VIEIRA LISBOA Patricia; ANGER-LEROY M.; MORNET Stephane; COLPO Pascal; ROSSI Francois|
|Citation:||Proceedings of SPIE-The International Society for Optical Engineering - ISSN 0277-786X vol. 7035|
|Publisher:||SPIE - The International Society for Optical Engineering|
|Type:||Articles in periodicals and books|
|Abstract:||A procedure for fabricating nanopatterned surfaces at the sub-500 nm scale comprising a hexagonal close packed array of bioadhesive gold nanoareas in a protein resistant matrix (PEO-like polymer), has been optimized. The surfaces were characterized by AFM analysis and their interaction with amino functionalised gold nanoparticles as models were investigated. The AFM images show the crystalline arrangement of nanopattern array and the localized adsorption of the H2N-Au nanoparticles in the bioadhesive nanoareas. A Surface Plasmon Resonance imaging (SPRi) system was used to assess the detection performances of these surfaces when employed as a transduction platform for studying biomolecule interactions. The investigated surfaces showed an enhancement of the affinity reaction efficiency with respect to the non structured surfaces. The obtained preliminary results show that nanostructuring the surfaces improve the binding site accessibility of the immobilized biological probes without significantly modifying the native biomolecule conformation.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
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