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dc.contributor.authorHEYRIES Kevinen_GB
dc.contributor.authorMANDON Celineen_GB
dc.contributor.authorCERIOTTI Lauraen_GB
dc.contributor.authorPONTI Jessicaen_GB
dc.contributor.authorCOLPO Pascalen_GB
dc.contributor.authorBLUM L.en_GB
dc.contributor.authorMARQUETTE Christopheen_GB
dc.date.accessioned2010-02-25T15:47:24Z-
dc.date.available2009-01-20en_GB
dc.date.available2010-02-25T15:47:24Z-
dc.date.created2009-01-20en_GB
dc.date.issued2009en_GB
dc.date.submitted2008-11-24en_GB
dc.identifier.citationBIOSENSORS & BIOELECTRONICS vol. 24 no. 5 p. 1146-1152en_GB
dc.identifier.issn0956-5663en_GB
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2008.06.042en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC48915-
dc.description.abstract"Macromolecules to PDMS transfer" technique relying on the direct entrapment of macromolecules spots during PDMS polymerisation is proposed as an alternative for the easy and simple PDMS surface modification. In the present work, the development of three different applications based on this procedure is presented as proof of the method potentialities. First, C-reactive protein (CRP) sandwich immunoassay using immobilised monoclonal anti-CRP antibodies was developed for sepsis diagnosis. The preserved integrity of the immobilised monoclonal immunoglobulin permitted the sensitive detection of free CRP in human sera (LOD= 12.5µg/L, detection ranging over two decades). Then, rheumatoid arthritis diagnosis through the rheumatoid factor (RF) detection based on rabbit immunoglobulins immobilisation allowed the detection of specific antibodies in human sera samples down to low RF levels (detection range 5.3-485 IU/mL). Finally, the "Macromolecules to PDMS transfer" procedure was used to easily and rapidly produce fibronectin-based cell culture arrays. The successful attachment of HeLa and BALB/3T3 cells was demonstrated with optical microscopy and specific staining of actin and vinculin.en_GB
dc.description.sponsorshipJRC.DDG.I.5-Nanobiosciencesen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherELSEVIER ADVANCED TECHNOLOGYen_GB
dc.relation.ispartofseriesJRC48915en_GB
dc.title"Macromolecules to PDMS Transfer" as a General Route for PDMS Biochipsen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.bios.2008.06.042en_GB
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