A method for the determination of acrylamide in roasted coffee was subjected to an inter-laboratory comparison study. The aim was to extend the scope of a method already standardised for the determination of acrylamide in bakery and potato products to include roasted coffee. Modifications of the already standardised method were therefore kept to a minimum.
The method was based on aqueous extraction of the roasted coffee matrix and solid phase extraction (SPE) clean-up followed by isotope dilution high performance liquid chromatography with tandem mass spectrometric detection (LC-MS/MS).
The test portion of the sample was spiked with stable isotope labelled acrylamide and extracted on a mechanical shaker with n-hexane and water for one hour. The sample extract was centrifuged, the organic phase was discarded, and a portion of the aqueous extract was further cleaned-up by solid phase extraction on an Isolute Multimode cartridge followed by a second clean-up step on an Isolute ENV+ cartridge. The volume of the acrylamide-containing fraction eluted from the second SPE column was reduced by evaporation and analysed by reverse-phase high performance liquid chromatography (HPLC) with tandem mass spectrometric detection.
Three coffee samples and one aqueous acrylamide standard solution were sent to 11 laboratories from 8 EU Member States. All samples were sent as blinded duplicates.
Based on the reported results the relative standard deviations for reproducibility (RSDR) were 11.5 % at a level of 160 µg/kg, 10.1 % at a level of 263 µg/kg, and 9.6 % at a level of 585 µg/kg. The values for repeatability (RSDr) in those materials ranged from 1 % to 3.5 %. The method performance parameters are satisfying with regard to internationally accepted criteria. Hence the method would be suitable for the enforcement of regulatory limits.
WENZL Thomas;
SZILAGYI Szilard;
ROSEN Johan;
KARASEK Lubomir;
2009-08-26
TAYLOR & FRANCIS LTD
JRC49105
0265-203X,
https://publications.jrc.ec.europa.eu/repository/handle/JRC49105,
10.1080/02652030902939689,
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