Single Molecule Detection in Nanofluidic Digital Array Enables Accurate Measurement of DNA Copy Number

Digital polymerase chain reaction (PCR) is a promising technique for estimating target DNA copy number. The DNA solution is distributed throughout a large number of partitions and then, following amplification, the target DNA copy number in the original solution is estimated based on the proportion of partitions containing amplified DNA. Random distribution of the target DNA molecules and single molecule amplification are critical to the validity of this approach. Here we use a certified plasmid calibrant containing two different target DNA sequences to identify approaches for obtaining the most reliable quantitative data from digital PCR. This fundamental study will increase the confidence of such measurements and advance the use of digital PCR for future bioanalytical research.

BHAT Somanath;  HERRMAN Jan;  ARMISHAW Paul;  CORBISIER Philippe;  EMSLIE K.; 

2009-04-27

SPRINGER HEIDELBERG

JRC49655

1618-2642,   

http://dx.doi.org/10.1007/s00216-009-2729-5,    https://publications.jrc.ec.europa.eu/repository/handle/JRC49655,   

10.1007/S00216-009-2729-5,