Title: Certification of a Reference Material with Candida Albicans (NCPF 3179) at a Target Level of 1000 Colony Forming Units per Material Sphere, IRMM-354
Authors: DE BAETS LiesbetMEEUS NeleSCHIMMEL Heinz
Publisher: OPOCE
Publication Year: 2009
JRC N°: JRC50066
ISBN: 978-92-79-11254-6
ISSN: 1018-5593
Other Identifiers: EUR 23707 EN
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC50066
DOI: 10.2787/19017
Type: EUR - Scientific and Technical Research Reports
Abstract: This report describes the certification of a reference material (IRMM-354) of Candida albicans. Certified Reference Materials (CRMs) for microbiological analysis are indispensible tools for development and validation of detection methods and for the implementation and support of internal and external quality control inthe area of microbiological analysis. Each vial of IRMM-354 contains one material sphere of Candida albicans. The homogeneity and stability (at -20 °C and -70 °C) of the batch was assessed by counting colony forming units (cfu) per material sphere on nutrient agar (NA) and Oxytetracyclin-Glucose-Yeast Extract (OGYE) agar. The material is stable when stored at a maximum temperature of -20 °C for up to 18 months. the batch was characterised by six laboratories to establish a certified value of cfu per material sphere on Na and OGYE agar. The certified value for NA is 917 cfu per material sphere with an expanded uncertainty of 168. The certified value for OGYE agar is 912 cfu per material sphere with an expanded uncertainty of 173. In both cases, a coverage factor k = 2 is used, corresponding to a level of confidence of about 95 %. The authenticity of the material was confirmed by DNA sequence analysis of the coding region for the hwpl gene.
JRC Directorate:Health, Consumers and Reference Materials

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