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dc.contributor.authorDE BAETS Liesbeten_GB
dc.contributor.authorMEEUS Neleen_GB
dc.contributor.authorSCHIMMEL Heinzen_GB
dc.date.accessioned2010-02-25T18:16:17Z-
dc.date.available2009-02-18en_GB
dc.date.available2010-02-25T18:16:17Z-
dc.date.created2009-02-18en_GB
dc.date.issued2009en_GB
dc.date.submitted2009-01-28en_GB
dc.identifier.isbn978-92-79-11254-6en_GB
dc.identifier.issn1018-5593en_GB
dc.identifier.otherEUR 23707 ENen_GB
dc.identifier.otherOPOCE LA-NA-23707-EN-Cen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC50066-
dc.description.abstractThis report describes the certification of a reference material (IRMM-354) of Candida albicans. Certified Reference Materials (CRMs) for microbiological analysis are indispensible tools for development and validation of detection methods and for the implementation and support of internal and external quality control inthe area of microbiological analysis. Each vial of IRMM-354 contains one material sphere of Candida albicans. The homogeneity and stability (at -20 °C and -70 °C) of the batch was assessed by counting colony forming units (cfu) per material sphere on nutrient agar (NA) and Oxytetracyclin-Glucose-Yeast Extract (OGYE) agar. The material is stable when stored at a maximum temperature of -20 °C for up to 18 months. the batch was characterised by six laboratories to establish a certified value of cfu per material sphere on Na and OGYE agar. The certified value for NA is 917 cfu per material sphere with an expanded uncertainty of 168. The certified value for OGYE agar is 912 cfu per material sphere with an expanded uncertainty of 173. In both cases, a coverage factor k = 2 is used, corresponding to a level of confidence of about 95 %. The authenticity of the material was confirmed by DNA sequence analysis of the coding region for the hwpl gene.en_GB
dc.description.sponsorshipJRC.D.2-Reference materialsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherOPOCEen_GB
dc.relation.ispartofseriesJRC50066en_GB
dc.titleCertification of a Reference Material with Candida Albicans (NCPF 3179) at a Target Level of 1000 Colony Forming Units per Material Sphere, IRMM-354en_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
dc.identifier.doi10.2787/19017en_GB
JRC Directorate:Health, Consumers and Reference Materials

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