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dc.contributor.authorSCARAVELLI Elenaen_GB
dc.contributor.authorBROHEE Marcelen_GB
dc.contributor.authorMARCHELLI Rosangelaen_GB
dc.contributor.authorVAN HENGEL Adrianusen_GB
dc.date.accessioned2010-02-25T15:12:31Z-
dc.date.available2009-08-13en_GB
dc.date.available2010-02-25T15:12:31Z-
dc.date.created2009-08-13en_GB
dc.date.issued2009en_GB
dc.date.submitted2009-03-11en_GB
dc.identifier.citationANALYTICAL AND BIOANALYTICAL CHEMISTRY vol. 395 p. 127-137en_GB
dc.identifier.issn1618-2642en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC50903-
dc.description.abstractPeanut allergic reactions can result from the ingestion of even very small quantities of peanut and represent a severe threat to the health of sensitised individuals. The detection of peanut traces in food products is therefore of prime importance. Peanut traces which can be (unintentionally) present in food products have usually undergone one or more processing steps like roasting and baking. Therefore, the methods designed to detect such traces have to be capable of detecting heat treated peanut. Commonly used methodologies designed to detect peanut traces in food products are enzyme-linked immunosorbent assays (ELISAs) that detect peanut specific proteins, and polymerase chain reaction (PCR) based methods targeting peanut specific DNA. A comparative analysis of such methods was performed and the impact of heat treatment on peanut kernels as well as the impact on a peanut-containing food matrix was investigated. Our results show that heat treatments have a detrimental effect on the detection of peanut with either type of method and that both types of methods are affected in a similar manner.en_GB
dc.description.sponsorshipJRC.D.8-Food safety and qualityen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherSPRINGER HEIDELBERGen_GB
dc.relation.ispartofseriesJRC50903en_GB
dc.titleThe Sffect of Heat Treatment on the Detection of Peanut Allergens as Determined by ELISA and Real-time PCRen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1007/s00216-009-2849-yen_GB
JRC Directorate:Health, Consumers and Reference Materials

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