Retention of Intratracheally Instilled and Ingested Tetravalent and Pentavalent Vanadium in the Rat

THE RETENTION, SUBCELLULAR DISTRIBUTION IN LIVER, KIDNEY AND LUNG AND BINDING OF VANADIUM IN THE RAT WERE INVESTIGATED AT 3H, 1D AND 12D AFTER INTRATRACHEAL INSTILLATION (IT.I.) OF 200 NG/KG B.W. OF 48-V- LABELLED PENTAVALENT AND TETRAVALENT VANADIUM. THE METABOLIC PATTERNS OF BOTH CHEMICAL FORMS OF VANADIUM WERE SIMILAR. LUNG, LIVER, KIDNEY, BONE, TESTIS AND SPLEEN ARE THE TARGET TISSUES AT RISK, VANADIUM BEING REMOVED WITH TIME AT DIFFERENT RATES. INTRACELLULARLY, THE MAJOR PART OF LIVER, KIDNEY AND LUNG VANADIUM WAS PRESENT IN THE NUCLEAR FRACTION (30 - 40% OF THE HOMOGENATE) FOLLOWED BY CYTOSOL AND MITOCHONDRIAL FRAC- TIONS. GEL FILTRATION CHROMATOGRAPHY OF THE LUNG CYTOSOL OBSERVED TWO BIOCHEMICAL POOLS OF VANADIUM THE FIRST CORRESPONDING TO PROTEIN-BOUND VANADIUM, THE SECOND POOL REPRESENTING A DIFFUSIBLE VANADIUM FORM. THE RETENTION OF THE TETRAVALENT AND PENTAVALENT VANADIUM SPECIES INVESTIGATED 1D AFTER PER OS (P.O) ADMINISTRATION SHOW NO OBVIOUS DIFFERENCES IN THE DISTRIBUTION PATTERN OF 48-V IN THE TISSUES, THE RETENTION FACTOR BEING HIGHER THAN TWO ORDERS OF MAGNITUDE IN COMPARISON WITH THE INTRACHEALLY INSTILLED ANIMALS. THESE FINDINGS SUGGEST THAT THE METABOLIC PATHWAYS OF TETRAVALENT AND PENTAVALENT VANADIUM ARE INDEPENDENT OF THE ROUTE OF VANADIUM EXPOSURE

SABBIONI Enrico;  EDEL Johanna; 

1995-03-15

JRC5115

https://publications.jrc.ec.europa.eu/repository/handle/JRC5115,