Novel Approach for Interlaboratory Transfer of Real-time PCR Methods: Detecting Bovine Meat and Bone Meal in Feed

The availability of robust methods for the species specific detection of meat and bone meal (MBM) in compound feedingstuffs, is an important prerequisite to enforce current and upcoming European legislation on the use of processed animal proteins in animal nutrition. Among possible methods, those based on DNA turned out to be a reliable tool for this aim, since DNA is a quite thermostable molecule able to resist severe heat treatments applied in the manufacturing of animal meals. The application of such methods by control laboratories implies that the method has been validated including an assessment of its robustness. Successful transferability between laboratories is considered an important robustness criterion of the method. However, corresponding guidelines regarding the design of such a study relevant to this field are missing. Here we demonstrate the feasibility of a new concept that was applied to check for the transferability of a qualitative assay for the detection of banned MBM in feedingstuffs at trace level based on real time PCR. This approach can be of interest for other research groups working in the development of real-time PCR methods and in their use by control laboratories.

PRADO RODRIGUEZ Marta;  FUMIERE O.;  BOIX SANFELIU Ana;  MARIEN Alin;  BERBEN Gilbert;  VON HOLST Christoph; 

2009-07-08

SPRINGER HEIDELBERG

JRC51447

1618-2642,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC51447,   

10.10007/s00216-009-2796-7,