Title: Influence of Baking Time and Matrix Effects on the Detection of Milk Allergens in Cookie Model Food System by ELISA
Authors: MONACI LindaBROHEE MarcelTREGOAT VirginieVAN HENGEL Adrianus
Citation: FOOD CHEMISTRY vol. 127 no. 2 p. 669-675
Publication Year: 2011
JRC N°: JRC51449
ISSN: 0308-8146
URI: www.elsevier.com/locate/foodchem
DOI: 10.1016/j.foodchem.2010.12.113
Type: Articles in periodicals and books
Abstract: Milk allergens are common allergens occurring in foods, therefore raising concern in allergic consumers. Enzyme-linked immunosorbent assay (ELISA) is, to date, the method of choice for the detection of food allergens by the food industry although, the performance of ELISA might be compromised when severe food processing techniques are applied to allergen-containing foods. In this paper we investigated the influence of baking time on the detection of milk allergens by using commercial ELISA kits. Baked cookies were chosen as a model food system and experiments were set up to study the impact of spiking a matrix food either before, or after the baking process. Results revealed clear analytical differences between both spiking methods, which stress the importance of choosing appropriate spiking methodologies for method validation purposes. Finally, since the narrow dynamic range of quantification of ELISA implies that dilution of samples is required, the impact of sample dilution on the quantitative results was investigated. All parameters investigated were shown to impact milk allergen detection by means of ELISA.
JRC Directorate:Health, Consumers and Reference Materials

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