Selection of chemicals for the development and evaluation of in vitro methods for skin sensitisation testing

Advances in the understanding of the biological mechanisms underlying skin sensitisation and the need to comply with recent regulatory requirements have favoured the development of alternative approaches some of which may provide promising screening or partial replacement methods. However, efforts are still required to identify more robust predictive endpoints and to further optimise existing methods to be integrated into a testing strategy in view of achieving full replacement of current animal tests. The European Centre for the Validation of Alternative Methods (ECVAM) and the European Cosmetics Toiletries and Perfumery Association (Colipa) have been collaborating with the aim of identifying a core set of reference chemicals to be proposed for use during test method development/optimisation. The use of a common set of reference chemicals in the development phase of methods would facilitate an early assessment of their performance with respect to existing tests and of their possible contribution to a testing strategy. By applying pre-defined criteria, existing databases were mined, and a list of 16 chemicals including 12 positive controls, of which 4 require metabolic activation to act as sensitisers and 4 negative controls, was identified. The chemicals and the criteria used for their inclusion into the list are presented here.

CASATI Silvia;  AEBY Pierre;  KIMBER Ian;  MAXWELL Gavin;  OVIGNE Jean Marc;  ROGGEN Erwin;  ROVIDA Costanza;  TOSTI Luca;  BASKETTER David; 

2009-12-07

FRAME

JRC51738

0261-1929,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC51738,