Optimisation and Validation of Programmed Temperature Vaporization (PTV) Injection in Solvent Vent Mode for the Analysis of the 15 + 1 EU-Priority PAHs by GC¿MS

This paper presents the optimisation of a programmed temperature vaporization solvent vent (PTV-SV) injection gas chromatographic mass spectrometric (GC-MS) method for the analysis of the 15 + 1 EU-priority PAHs in food extracts. Three operation parameters (vent time, vent flow and vent pressure) were optimised by applying a D-optimal experimental design. Among these variables, vent time showed the highest effect on the analytical response (signal intensity) of the target PAHs. The 15 + 1 EU-priority PAHs were analysed in solvent solutions and in extracts of fortified sausage. In addition, blank lamb meat extracts were prepared and spiked with the target PAHs prior to GC-MS analysis. The performance of the optimised PTV-SV injection GC-MS method was scrutinised for linearity, precision, matrix effects and robustness. All parameters were found satisfactorily. Compared to PTV injection in splitless mode, the PTV-SV injection method provided an enhancement of sensitivity for all target PAHs. Especially significant was the improvement of the S/N ratios of the compounds with the highest molecular mass.

GOMEZ RUIZ Jose Angel;  CORDEIRO RAPOSO Fernando;  LOPEZ SANCHEZ Patricia;  WENZL Thomas; 

2009-10-20

ELSEVIER SCIENCE BV

JRC52353

0039-9140,   

www.elsevier.com/locate/talanta,    https://publications.jrc.ec.europa.eu/repository/handle/JRC52353,   

10.1016/j.talanta.2009.07.041,