Patterned Growth and Differentiation of Human Cord Blood-Derived Neural Stem Cells on Bio-Functionalized Surfaces

Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells' development in the large scale multiparameter biotests.

BUZANSKA Leonora;  RUIZ Ana;  ZYCHOWICZ Marzena;  RAUSCHER Hubert;  CERIOTTI Laura;  ROSSI Francois;  COLPO Pascal;  DOMANSKA-JANIK Krystyna;  COECKE Sandra; 

2010-01-28

NENCKI INST EXPERIMENTAL BIOLOGY

JRC54465

0065-1400,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC54465,