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|Title:||Patterned Growth and Differentiation of Human Cord Blood-Derived Neural Stem Cells on Bio-Functionalized Surfaces|
|Authors:||BUZANSKA Leonora; RUIZ Ana; ZYCHOWICZ Marzena; RAUSCHER Hubert; CERIOTTI Laura; ROSSI Francois; COLPO Pascal; DOMANSKA-JANIK Krystyna; COECKE Sandra|
|Citation:||ACTA NEUROBIOLOGIAE EXPERIMENTALIS vol. 69 no. 1 p. 24-36|
|Publisher:||NENCKI INST EXPERIMENTAL BIOLOGY|
|Type:||Articles in periodicals and books|
|Abstract:||Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells' development in the large scale multiparameter biotests.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
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