Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing
cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells' development in the large scale multiparameter biotests.
BUZANSKA Leonora;
RUIZ Ana;
ZYCHOWICZ Marzena;
RAUSCHER Hubert;
CERIOTTI Laura;
ROSSI Francois;
COLPO Pascal;
DOMANSKA-JANIK Krystyna;
COECKE Sandra;
2010-01-28
NENCKI INST EXPERIMENTAL BIOLOGY
JRC54465
0065-1400,
https://publications.jrc.ec.europa.eu/repository/handle/JRC54465,
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