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dc.contributor.authorALONSO-SALCES Rosaen_GB
dc.contributor.authorGUILLOU Claudeen_GB
dc.contributor.authorBERRUETA Luis A.en_GB
dc.identifier.citationRAPID COMMUNICATIONS IN MASS SPECTROMETRY vol. 23 p. 363-383en_GB
dc.description.abstractLiquid chromatography coupled with a photodiode array detector, electrospray ionization, collision- induced dissociation and tandem mass spectrometry (LC-DAD/ESI-CID-MS/MS) on a triple quad- rupole (QqQ) has been used to detect and characterize polyphenols and methylxanthines in green coffee beans: three phenolic acids (caffeic acid, ferulic acid and dimethoxycinnamic acid), three isomeric caffeoylquinic acids (Mr 354), three feruloylquinic acids (Mr 368), one p-coumaroylquinic acid (Mr 338), three dicaffeoylquinic acids (Mr 516), three feruloyl-caffeoylquinic acids (Mr 530), four p-coumaroyl-caffeoylquinic acids (Mr 500), three diferuloylquinic acids (Mr 544), six dimethoxycin- namoyl-caffeoylquinic acids (Mr 544), three dimethoxycinnamoyl-feruloylquinic acids (Mr 558), six cinnamoyl-amino acid conjugates, three cinnamoyl glycosides, and three methylxanthines (caffeine, theobromine and theophylline). Dimethoxycinnamic acid, three isomers of dimethoxycinnamoyl- caffeoylquinic acids and another three of dimethoxycinnamoyl-feruloylquinic acids, as well as the three cinnamoyl glycosides, had not previously been reported in coffee beans. Structures have been assigned on the basis of the complementary information obtained from UV-visible spectra, relative hydrophobicity, scan mode MS spectra, and fragmentation patterns in MS2 spectra (both in the positive and negative ion modes) obtained using a QqQ at different collision energies. A structure diagnosis scheme is provided for the identification of different isomers of polyphenols and methylxanthines.en_GB
dc.description.sponsorshipJRC.I.6-Systems toxicologyen_GB
dc.publisherJOHN WILEY & SONS LTDen_GB
dc.titleLiquid chromatography coupled with ultraviolet absorbance detection, electrospray ionization, collision- induced dissociation and tandem mass spectrometry on a triple quadrupole for the on-line characterization of polyphenols and methylxanthines in green coffee beansen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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