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dc.contributor.authorMAZZARA Marcoen_GB
dc.contributor.authorFOTI Nicolettaen_GB
dc.contributor.authorSAVINI Cristianen_GB
dc.contributor.authorBONFINI Lauraen_GB
dc.contributor.authorVAN DEN EEDE Guyen_GB
dc.date.accessioned2010-04-19T15:09:33Z-
dc.date.available2010-02-12en_GB
dc.date.available2010-04-19T15:09:33Z-
dc.date.created2010-02-10en_GB
dc.date.issued2009en_GB
dc.date.submitted2010-01-14en_GB
dc.identifier.isbn978-92-79-14981-8en_GB
dc.identifier.issn1018-5593en_GB
dc.identifier.otherEUR 24236 ENen_GB
dc.identifier.otherOPOCE LB-NA-24236-EN-Cen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC56610-
dc.description.abstractThe JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a qualitative event-specific method to detect the AG3139 transformation event in bacterial biomass derived from E. coli K-12 DNA. The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 ¿on genetically modified food and feed¿ and with Regulation (EC) No 641/2004 of 6 April 2004 ¿on detailed rules for the implementation of Regulation (EC) No 1829/2003¿, Ajinomoto Eurolysine S.A.S. provided the detection method and the samples (genomic DNA from E. coli K-12 harbouring the AG3139 event, bacterial biomass derived thereof, genomic DNA from the parental organism E. coli K-12 MG1655, and plasmids as positive and negative control samples). The JRC prepared the validation samples. The collaborative trial involved twelve laboratories from eight European countries. The results of the international collaborative trial met the ENGL performance requirements (see Annex 1). The method is, therefore, considered applicable to the control and feed samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004. The results of the collaborative study are made publicly available at http://gmo-crl.jrc.ec.europa.eu/.en_GB
dc.description.sponsorshipJRC.DDG.I.4-Molecular biology and genomicsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherPublications Office of the European Unionen_GB
dc.relation.ispartofseriesJRC56610en_GB
dc.titleEvent-specific Method for the Detection of Dried-killed Bacterial Biomass PT73 (TM) Derived from E. coli GM Strain AG3139 Using Real-time PCRen_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
dc.identifier.doi10.2788/58900en_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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