X-Ray Spectromicroscopy Study of Ubiquitin Adsorption to Plasma Polymerized Microstructures
Synchrotron-based X-ray PhotoEmission Electron Microscopy (X-PEEM), Atomic Force Microscopy (AFM), time of flight secondary ion mass spectrometry (ToF SIMS) and X-ray photoelectron spectroscopy (XPS) have been used to visualize and characterize plasma polymerized acrylic acid (ppAA) and poly ethylene oxide (PEO)-like microstructures. Micropatterned microstructures have been fabricated with electron-beam lithography to obtain contrasted ppAA/PEO-like structures. A surface area of 500 µm× 500 µm was patterned with arrays of 5 µm diameter circles and 500 µm x 5 µm lines. Samples have been analyzed before and after immersion of ubiquitin protein solutions.
AFM and X-PEEM results indicated that proteins adsorbed preferentially on the ppAA regions. For both circular and line patterns, the adsorbed protein preferentially adsorbed to the ppAA regions.
CECCONE Giacomo;
LEUNG B.;
PEREZ ROLDAN Maria;
VALSESIA Andrea;
COLPO Pascal;
ROSSI Francois;
HITCHCOCK A.;
SCHOLL A.;
2010-07-20
JOHN WILEY & SONS LTD
JRC56704
0142-2421,
www.interscience.wiley.com,
https://publications.jrc.ec.europa.eu/repository/handle/JRC56704,
10.1002/sia.3239,
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