Full metadata record
DC FieldValueLanguage
dc.contributor.authorVIEIRA LISBOA Patriciaen_GB
dc.date.accessioned2010-05-28T14:18:40Z-
dc.date.available2010-01-29en_GB
dc.date.available2010-05-28T14:18:40Z-
dc.date.created2010-01-28en_GB
dc.date.issued2009en_GB
dc.date.submitted2010-01-27en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC56913-
dc.description.abstractSensors based on biomolecular recognition, i.e. biosensors, are very promising tools for the development of advanced analytical devices. Among all techniques available, Surface Plasmon Resonance is a label-free optical technique that allows quantitative analysis in real time and provides outstanding detection performances. The improvement of biosensor performances, such as sensitivity, specificity and cross reaction control, is intrinsically related to the quality of the bio-interface. Bio-interface is the location where the biomolecules interact with the surface and where the biomolecular recognition process occurs. The main challenge is to design a well controlled bio-interface that allows the immobilisation of the bio-probes in an active state to promote efficient biomolecular recognition. A recent promising approach to improve the detection of biomolecular recognition concerns the creation of surfaces with chemical nano-contrasts (for instance adhesives/non adhesives) to reduce steric hindrance and provide a better availability of the binding sites. In this thesis, we describe the fabrication, by plasma colloidal lithography, of nanoarrayed surfaces that induce this effect. Moreover it is shown that an optical enhancement of the signal due to the crystalline geometry of the features creates unique optical characteristics related to surface plasmons excitation. These nanoarrays were applied to SPR detection of several immunoreactions demonstrating that nano-patterned surfaces with optimal geometry improve significantly the SPR detection sensitivity.en_GB
dc.description.sponsorshipJRC.DG.I.5-Nanobiosciencesen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.relation.ispartofseriesJRC56913en_GB
dc.titleDevelopment of Nanoarrayed Surfaces for Improved SPR Detection of Biomolecular Recognitionen_GB
dc.typePhD Thesesen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.