Report on the Verification of the Performance of MON89034 and MON88017 Event-specific Methods on the Maize Event MON89034 x MON88017 Using Real-Time PCR
The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established
by Regulation (EC) No 1829/2003, has carried out an in-house verification study to assess the
performance of two quantitative event-specific methods on the maize event MON89034 x
MON88017 (unique identifier MON-89Ø34-3 x MON-88Ø17-3) which combines the MON89034
and MON88017 transformation events. The two methods have been validated individually on
single-trait events, to detect and quantify each event in maize samples. This study was
conducted according to internationally accepted guidelines (1, 2).
In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified
food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the
implementation of Regulation (EC) No 1829/2003, Monsanto Company provided the detection
methods and the control samples: genomic DNA extracted from seeds of MON89034 x
MON88017 maize, genomic DNA extracted from seeds of non-GM maize, seeds of MON89034 x
MON88017 and seed of conventional maize. The JRC prepared the in-house verification
samples (calibration samples and blind samples at different GM percentages).
The results of the in-house verification study were evaluated with reference to ENGL method
performance requirements (http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm) and to the
validation results on the parental events (http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm).
The results of this CRL-GMFF in-house verification studies are made publicly available at
http://gmo-crl.jrc.ec.europa.eu/
CHARLES DELOBEL Chrystele;
GRAZIOLI Emanuele;
MAZZARA Marco;
VAN DEN EEDE Guy;
2010-03-03
Publications Office of the European Union
JRC57222
978-92-79-15221-4,
1018-5593,
EUR 24276 EN,
OP LB-NA-24276-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC57222,
10.2788/71542,
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