The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)

Seven international laboratories specializing in the determination of marine pigment concentrations using high performance liquid chromatography (HPLC) were intercompared using in situ samples and a mixed pigment sample. The field samples were collected primarily from oligotrophic waters, although mesotrophic and eutrophic waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two orders of magnitude (0.020-1.366 mg m^-3). The intercomparisons were used to establish the following: a) the uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b) the reduction in uncertainties as a result of applying quality assurance (QA) procedures; c) the importance of establishing a properly defined referencing system in the computation of uncertainties; d) the analytical benefits of performance metrics, and e) the utility of a laboratory mix in understanding method performance. In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated to determine whether or not the average uncertainty for this measurement is being satisfied.

HOOKER Stanford;  VAN HEUKELEM Laurie;  THOMAS Crystal;  CLAUSTRE Hervé;  RAS Joséphine;  SCHLUTER Louise;  CLEMENTSON Lesley;  VAN DER LINDE Dirk;  EKER-DEVELI Elif;  BERTHON Jean-Francois;  BARLOW Ray;  SESSIONS Heather;  ISMAIL Hassan;  PERL Jason; 

2010-03-23

Goddard Space Flight Center

JRC57305