The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)
Seven international laboratories specializing in the determination of marine pigment concentrations using high
performance liquid chromatography (HPLC) were intercompared using in situ samples and a mixed pigment
sample. The field samples were collected primarily from oligotrophic waters, although mesotrophic and eutrophic
waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two
orders of magnitude (0.020-1.366 mg m^-3). The intercomparisons were used to establish the following: a) the
uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b)
the reduction in uncertainties as a result of applying quality assurance (QA) procedures; c) the importance
of establishing a properly defined referencing system in the computation of uncertainties; d) the analytical
benefits of performance metrics, and e) the utility of a laboratory mix in understanding method performance.
In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated
to determine whether or not the average uncertainty for this measurement is being satisfied.
HOOKER Stanford;
VAN HEUKELEM Laurie;
THOMAS Crystal;
CLAUSTRE Hervé;
RAS Joséphine;
SCHLUTER Louise;
CLEMENTSON Lesley;
VAN DER LINDE Dirk;
EKER-DEVELI Elif;
BERTHON Jean-Francois;
BARLOW Ray;
SESSIONS Heather;
ISMAIL Hassan;
PERL Jason;
2010-03-23
Goddard Space Flight Center
JRC57305
Additional supporting files
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