A rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision tool for adventitious genetically modified (GM) presence (AGMP) has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction (PCR) method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Pre-validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSDr = 18.5%; RSDR = 32.8; Bias = 26.7%).
LANGRELL Stephen;
ALLNUT Theodore;
LAVAL Valerie;
BERTHEAU Yves;
PLA Maria;
PAPAZOVA Nina;
DAVID Lee;
2011-09-08
SPRINGER
JRC57766
1936-9751,
http://www.springerlink.com/content/d214w0t438h77271/,
https://publications.jrc.ec.europa.eu/repository/handle/JRC57766,
10.1007/s12161-010-9167-z,
| Name | Country | City | Type |
|---|
This document is only visible at the Commission level.
You are not authorized to publish or distribute it outside the European Commission.
This is a public document. You can share this publication.
Datasets
| ID | Title | Public URL |
|---|
Dataset collections
| ID | Acronym | Title | Public URL |
|---|
Scripts / source codes
| Description | Public URL |
|---|
Additional supporting files
| File name | Description | File type |
|---|