Validation of a Real-time PCR On-Site Quantification Method for MON810 Maize

A rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision tool for adventitious genetically modified (GM) presence (AGMP) has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction (PCR) method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Pre-validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSDr = 18.5%; RSDR = 32.8; Bias = 26.7%).

LANGRELL Stephen;  ALLNUT Theodore;  LAVAL Valerie;  BERTHEAU Yves;  PLA Maria;  PAPAZOVA Nina;  DAVID Lee; 

2011-09-08

SPRINGER

JRC57766

1936-9751,   

http://www.springerlink.com/content/d214w0t438h77271/,    https://publications.jrc.ec.europa.eu/repository/handle/JRC57766,   

10.1007/s12161-010-9167-z,