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dc.contributor.authorLANGRELL Stephenen_GB
dc.contributor.authorALLNUT Theodoreen_GB
dc.contributor.authorLAVAL Valerieen_GB
dc.contributor.authorBERTHEAU Yvesen_GB
dc.contributor.authorPLA Mariaen_GB
dc.contributor.authorPAPAZOVA Ninaen_GB
dc.contributor.authorDAVID Leeen_GB
dc.date.accessioned2011-09-09T00:00:51Z-
dc.date.available2011-09-08en_GB
dc.date.available2011-09-09T00:00:51Z-
dc.date.created2011-08-25en_GB
dc.date.issued2011en_GB
dc.date.submitted2010-03-23en_GB
dc.identifier.citationFOOD ANALYTICAL METHODS vol. 4 no. 3 p. 313-318en_GB
dc.identifier.issn1936-9751en_GB
dc.identifier.urihttp://www.springerlink.com/content/d214w0t438h77271/en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC57766-
dc.description.abstractA rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision tool for adventitious genetically modified (GM) presence (AGMP) has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction (PCR) method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Pre-validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSDr = 18.5%; RSDR = 32.8; Bias = 26.7%).en_GB
dc.description.sponsorshipJRC.J.5-Agriculture and Life Sciences in the Economyen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherSPRINGERen_GB
dc.relation.ispartofseriesJRC57766en_GB
dc.titleValidation of a Real-time PCR On-Site Quantification Method for MON810 Maizeen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1007/s12161-010-9167-zen_GB
JRC Directorate:Growth and Innovation

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