Report of the 2009 Proficiency Test of the Community Reference Laboratory for Mycotoxins, for the Network of National Reference Laboratories, Regarding the Determination of T-2 and HT-2 Toxins in Cereal Products
A proficiency test was conducted by the Community Reference Laboratory for Mycotoxins with 29 European National Reference Laboratories (NRLs) for Mycotoxins and one laboratory from a candidate country. The materials shipped were a solution of known T-2 and HT-toxins content in acetonitrile and three cereal test materials with unknown levels of T-2 and HT-2 toxins. Laboratories determined the content of T-2 and HT-toxins mainly by either enzyme linked immuno-sorbent assay (ELISA), gas chromatography (GC-MS) or high-performance liquid-chromatography (HPLC) followed by fluorescence or mass selective detection (MS). From each Member State (MS) one NRL reported results, with two MS reporting results from two different NRLs, one for feed an one for food.
Applying the Horwitz equation as a basis for setting the target standard deviation for proficiency (19.6% for T-2 toxin and 21.5% for HT-2 toxin in the spiked test material), resulted in 21 out of the 30 laboratories reporting satisfactory z-scores for T-2 toxins and 15 laboratories reporting satisfactory ones for HT-2 toxins after recovery correction. Two laboratories did not send in results for HT-2 toxin, but only for T-2 toxin. Four laboratories reported questionable results within a z-score limit of 2 to 3 for T-2 toxin and 2 laboratories for HT-2 toxin. The remaining laboratories reported z-scores above 3, which are unsatisfactory. Taking the ¿-score as benchmark for the combined parameter (T 2 & HT 2 toxin) the number of satisfactory results reduced to 14. No z scores were calculated for the low contaminated and the high contaminated material.
STROKA Joerg;
BREIDBACH Andreas;
BOUTEN Katrien;
KROEGER-NEGOITA Katy;
AMBROSIO Massimo;
LERDA Donata;
2010-03-31
Publications Office of the European Union
JRC57772
978-92-79-15498-0,
1018-5593,
EUR 24315 EN,
OP LA-NA-24315-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC57772,
10.2787/24867,
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