Title: Constant Neutral Loss Spectra of Small Peptides
Authors: PELZER G.DE PAUW EdwinNASAR K.
Citation: Biomedical Environmental Mass Spectrometry vol. 15 p. 577-581
Publication Year: 1988
JRC N°: JRC5819
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC5819
Type: Articles in periodicals and books
Abstract: PROGRAMS HAVE BEEN DEVELOPED TO HELP OR AUTOMIZE SPECTRA ANALYSIS. THEY ARE BASED ON EXACT MEASUREMENTS AND SPECTRA FITTING BUT THE RAPIDLY GROWING NUMBER OF RESIDUE PERMUTATIONS LIMITS THEIR USE. WE ADOPTED A STRATEGY DESIGNED TO REDUCE THE NUMBER OF ALLOWED SOLUTIONS OWING TO AN INCREASE IN MASS SPECTROMETRIC INFORMATION. WE TRIED TO IDENTIFY UNAMBIGUOUS SEQUENCE INFORMATION, SPECIFIC N- OR C-TERMINAL FRAGMENTS, WITHOUT SAMPLE MODIFICATIONS. METASTABLE TRANSITIONS WERE MINOTIRED USING THE CONSTANT NEUTRAL LOSS LINKED SCAN (CNL) MODE. RESULTS WERE COMPARED TO THOSE OBTAINED IN A PURE MS-MS MODE USING A HYBRID INSTRUMENT.
JRC Directorate:Joint Research Centre Historical Collection

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