Virgin Olive Oil Authentication by Multivariate Analyses of 1H NMR Fingerprints and delta 13C and delta 2H Data

1H NMR fingerprints of virgin olive oils (VOOs) from the Mediterranean basin (three harvests) were analyzed by principal component analysis, linear discriminant analysis (LDA), and partial least-squares discriminant analysis (PLS-DA) to determine their geographical origin at the national, regional, or PDO level. Further delta 13C and delta 2H measurements were performed by isotope ratio mass spectrometry (IRMS). LDA and PLS-DA achieved consistent results for the characterization of PDO Riviera Ligure VOOs. PLS-DA afforded the best model: for the Liguria class, 92% of the oils were correctly classified in the modeling step, and 88% of the oils were properly predicted in the external validation; for the non-Liguria class, 90 and 86% of hits were obtained, respectively. A stable and robust PLS-DA model was obtained to authenticate VOOs from Sicily: the recognition abilities were 98% for Sicilian oils and 89% for non-Sicilian ones, and the prediction abilities were 93 and 86%, respectively. More than 85% of the oils of both categories were properly predicted in the external validation. Greek and non-Greek VOOs were properly classified by PLS-DA: >90% of the samples were correctly predicted in the cross-validation and external validation. Stable isotopes provided complementary geographical information to the 1H NMR fingerprints of the VOOs.

ALONSO-SALCES Rosa;  MORENO-ROJAS Jose Manuel;  HOLLAND Margaret;  RENIERO Fabiano;  GUILLOU Claude;  HEBERGER Karoly; 

2011-01-19

AMER CHEMICAL SOC

JRC58393

0021-8561,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC58393,   

10.1021/jf903989b,