Determination of Fumonisins B1 and B2 in Corn by LC/MS with Immunoaffinity Column Cleanup: Interlaboratory Study

An interlaboratory validation study was conducted to establish the method performance characteristics of an immunoaffinity column (IAC) cleanup procedure followed by LC/MS for the determination of fumonisins B1 (FB1) and B2 (FB2) and combined FB1 + FB2 in corn. Naturally contaminated corn samples were milled to a fine powder and mixed to produce three samples with target levels of combined FB1 + FB2 ranging from 350 to 4000 µg/kg. Of 15 initially participating laboratories, two failed to report results and another did not follow the prescribed method. Thus, valid results were obtained from 12 participants located in 11 countries. Statistical analysis of the results produced RSDr values of 4.6-11.9, 1.9-12.6, and 1.4-11.5% for FB1, FB2, and combined FB1 + FB2, respectively; the corresponding RSDR values were 19.8-23.8, 18.2-25.5, and 18.8-23.2%. The three concentration levels of combined FB1 + FB2 were 534, 1194, and 1954 µg/kg. HorRat values for r and R were all <2.0, indicating that the method is suitable as a regulatory method for the enforcement of European Union limits for fumonisins in corn.

SENYUVA Hamide;  GILBERT John;  STROKA Joerg; 

2010-10-04

AOAC INT

JRC58887

1060-3271,