Chapter 4: Towards reference materials as a tool for validating results of MBM in animal feed

In order to ensure the quality of data obtained from control laboratories measuring meat and bone meal (MBM) in feed, IRMM has developed concepts and starting materials for the possible production of reference materials in this field. The measurements to be verified are both qualitative and quantitative to their nature. Two main routes have been tested for the development of such materials, namely dry-mixing of bulk materials and direct gravimetric preparation of individual samples. The first approach consisted of addition of known amounts of meat and bone powders to a maize powder and subsequent 3-dimensional mixing. The resulting powder was then split up in different sample containers and checked for homogeneity. The second approach consisted of a production with exactly known masses of meat and bone meal powder added to a standard background matrix of maize powder prepared directly in each jar. The latter samples could only be used in single-shot analysis. The existing official method (as described in Annex VI of Commission Regulation EC 152/2009) based on sedimentation and light microscopy was used for evaluation of materials as well as NIR microscopy and PCR. This chapter describes the concept, raw materials, the two different approaches for preparation and analytical results. The results are promising in so far that the best set of results obtained for the samples prepared by direct gravimetric preparation were within 10 % of the known target with a precision of ±11 % RSD using the official EU-method. Possible routes for future material preparation and measurement developments are also discussed.

CHAROUD-GOT Jean;  TUMBA-TSHILUMBA Marie-France;  BAETEN Vincent;  VANCUTSEM Jeroen;  EMTEBORG Per; 

2013-02-28

Les Editions namuroises

JRC58893

978-2-87551-029-7,