Preparation of VtiO2 labelled nanoparticles for biokinetics studies

Engineered nanoparticles are of current interest in many applications (e.g. industry, medicine, energy). As they are produced in ever higher quantities, the need to assess their biokinetics and toxicity becomes more and more important. The aim of this study was to prepare stable, radiolabelled bioavailable TiO2 nanoparticles to assess their in vivo biokinetics. The preparation of [V-48]TiO2 nanoparticles by direct proton irradiation is described. Irradiations were performed on the Scanditronix MC-40 cyclotron at JRC Ispra, Italy with a 5 µA proton beam of 23.5 MeV energy. After 16 hours irradiation, a commercial sample of TiO2 (ST-01, Ishihara Corp. Japan) yielded approx. 40 MBq of V-48 activity at EOB. Half of the irradiated material was processed two weeks after EOB to size-select and purify the labelled nanoparticles from free V-48 by centrifugation and filtration. This yielded 855 kBq of final suspension of fine [V-48]TiO2 labeled nanoparticles (Z average = 105 nm) without free V-48. In vivo results in a rat model indicated good V-48 label stability with less than 1% of the administered dose eliminated by renal excretion, compared to ionic V-48 with approximately 50% renal clearance.

GIBSON Peter;  KOZEMPEL Jan;  ABBAS Kamel;  SIMONELLI Federica;  CYDZIK Izabela;  HOLZWARTH Uwe;  KREYLING Wolfgang;  SEMMLER-BEHNKE Manuela;  WENK A.;  DAWSON Kenneth;  RAMIREZ-GARCIA S.; 

2010-07-13

CHEMICKE LISTY

JRC59055

0009-2770,   

http://www.chemicke-listy.cz/docs/full/2010_14_s212-s221.pdf,    https://publications.jrc.ec.europa.eu/repository/handle/JRC59055,