A method for the determination of fumonisin B1 (FB1) and B2 (FB2) in different commercial maize-based products for infants and young children was developed and tested in a limited validation study involving 3 laboratories. The method used extraction at 55C with an acidic mixture of methanol-acetonitrile-citrate/phosphate buffer, clean-up through immunoaffinity column and fumonisin determination by high performance liquid chromatography with automated pre-column derivatisation with 0-phthaldialdehyde. Recovery experiments were performed at five spiking levels in the ranges of 80-800 ug/kg FB1 and 20-200 ug/kg FB2.
Mean recoveries ranged from 83 to 97% for PB1 and from 61-78% for FB2. Relative standard deviations for within-laboratory repeatability (RSDr) ranged from 5 to 12% for PFb and from 8-13% for FB2, whereas relative standard deviation for between-laboratory reproducibility (RSDR) ranged from 6 to 10% for FB1 and from 9-16% for FB2. The limit of quantification of the method (signal to noise ratio of 60 was 2.8 ug/kg for FB1 and 2.2 ug/kg for FB2.
Fumonisins were found in 6 out of 19 maize-based baby foods obtained from the Italian retail market at levels up to 53 ug/kg.
DE GIROLAMO A.;
PEREBOOM-DE FAUW D.;
SIZOO E.;
VAN EGMOND Hans;
GAMBACORTA L.;
BOUTEN Katrien;
STROKA Joerg;
VISCONTI A.;
SOLFRIZZO M.;
2010-08-03
Wageningen Academic Publishers
JRC59074
1875-0710,
http://wageningenacademic.metapress.com/content/k300856634842774/,
https://publications.jrc.ec.europa.eu/repository/handle/JRC59074,
10.3920/WMJ2010.1213,
| Name | Country | City | Type |
|---|
This document is only visible at the Commission level.
You are not authorized to publish or distribute it outside the European Commission.
This is a public document. You can share this publication.
Datasets
| ID | Title | Public URL |
|---|
Dataset collections
| ID | Acronym | Title | Public URL |
|---|
Scripts / source codes
| Description | Public URL |
|---|
Additional supporting files
| File name | Description | File type |
|---|