Development of a Recombinant Fab-Fragment Based Electrochemical Immunosensor for Deoxynivalenol Detection in Food Samples

A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab-fragment is presented. The analytical system employed. Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers. Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC50 of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab-fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analysing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products.

ROMANAZZO Daniela;  RICCI Francesco;  VOLPE Giulia;  ELLIOTT Christopher T.;  VESCO Silvia;  KROEGER-NEGOITA Katy;  MOSCONE Danila;  STROKA Joerg;  VAN EGMOND Hans;  VEHNIAINEN Markus;  PALLESCHI Giuseppe; 

2010-10-11

ELSEVIER ADVANCED TECHNOLOGY

JRC59484

0956-5663,   

www.elsevier.com/locate/bios,    https://publications.jrc.ec.europa.eu/repository/handle/JRC59484,   

10.1016/j.bios.2010.04.029,