Validation of Dynamic Light Scattering and Centrifugal Liquid Sedimentation Methods for Nanoparticle Characterisation

A variety of techniques exists to analyse the size and size distribution of nanoparticles in a suspension. However, neither full method validation reports nor suitable reference materials with properly assigned values traceable to SI are easily found in the literature or on the market, respectively. This paper presents results of in-house validation studies of Dynamic Light Scattering (DLS) and Differential Centrifugal Sedimentation (DCS) methods. Repeatability and intermediate precision were assessed using the IRMM 304 reference Materials (silica nanoparticles suspended in an aqueous solution) to estimate the uncertainty contribution from within-day or day-to-day variation effects. Relative standard and expanded uncertainties were calculated with the goal to obtain a reliable measurement uncertainty valid for measuring silica nanoparticles of for particles with similar density and size range. The validated methods will be used in the certification of nanoparticle reference materials.

BRAUN Adelina;  COUTEAU Olivier;  FRANKS Katrin;  KESTENS Vikram;  ROEBBEN Gert;  LAMBERTY Marie Andree;  LINSINGER Thomas; 

2011-10-20

ELSEVIER SCIENCE BV

JRC59767

0921-8831,   

www.elsevier.com/locate/apt,    https://publications.jrc.ec.europa.eu/repository/handle/JRC59767,   

10.1016/j.apt.2010.11.001,