Inflammation and reactive oxygen/nitrogen species in glial/neuronal cultures
Inflammation contributes to a wide variety of brain pathologies. In this chapter methods are described for using microglia and astrocytes in culture to investigate inflammatory processes and inflammatory neurodegeneration, including the use of neuronal/glial co-cultures and transwells. Methods for detecting and characterising inflammatory activation in culture, including changes in microglial phenotype,microglial phagocytosis and expression of pro-inflammatory cytokines . The protocols for how to determine cellular production of superoxide, hydrogen peroxide, peroxynitrite and nitric oxide in culture are described.
NEHER Jonas J.;
BROWN Guy C.;
KINSNER-OVASKAINEN Agnieszka;
PRICE Anna;
2012-01-09
Springer
JRC59783
978-1-61779-076-8,
0893-2336,
https://publications.jrc.ec.europa.eu/repository/handle/JRC59783,
10.1007/978-1-61779-077-5_16,
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