Inter-laboratory Comparison of an Analytical Method for the Determination of the Feed Additive Semduramicin in Poultry Feed at Authorised Level by Liquid Chromatography Single Quadrupole Mass Spectrometry

A collaborative study has been carried out according to international recognized guidelines in order to establish the performance characteristics of a liquid chromatography single quadrupole mass spectrometry analytical method for the determination of the feed additive semduramicin (SEM) in poultry feed at the authorized level (20-25 mg kg-1) valid within the European Union. Fifteen laboratories went through the validation phase of the study and all of them reported results. The content of SEM in the tested materials, provided as blind duplicates, ranged from 11.5 mg kg-1, which corresponds to half of the mean authorized level to 45.0 mg kg-1 which corresponds to twice the mean authorized level. All the materials have been analyzed by the participating laboratories using two different quantification approaches, namely standard addition and external standard calibration. The relative standard deviation of reproducibility (RSDR) for both quantification approaches varied from 8 to 18 %, corresponding to HORRAT values ranging from 0.8 to 1.5, which were therefore in all cases below the critical value of 2.0. Consequently the proposed analytical method and both quantification approaches can be considered fully validated and transferable to the control laboratories to be applied for the determination of SEM in poultry compound feed at authorized level within the frame of official control. Further steps of the administrative procedure aimed to the adoption of the method as part of an ISO/CEN standard are currently ongoing.

GONZALEZ DE LA HUEBRA Maria Jose;  VINCENT Ursula;  VON HOLST Christoph; 

2011-01-25

TAYLOR & FRANCIS LTD

JRC60760

0265-203X,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC60760,   

10.1080/19440049.2010.538727,