Please use this identifier to cite or link to this item:
|Title:||Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence|
|Authors:||BEDNARKIEWICZ Artur; RODRIGUES Robim; WHELAN Maurice|
|Citation:||TOXICOLOGY IN VITRO vol. 25 no. 8 p. 2088–2094|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Type:||Articles in periodicals and books|
|Abstract:||A quantitative, non-destructive cellular autofluorescence based in vitro imaging assay has been developed and applied to study the cytotoxicity of Sodium Lauryl Sulfate (SLS) and HgCl2 chemical compounds on Balb 3T3 cell culture. A phenomenological double logistic model was proposed to quantify and relate the observed kinetic changes of fluorescence to the toxic potency of chemical compounds. This work forms the basis to use cellular autofluorescence measurements in the in vitro toxicity screening assays. Keywords autofluorescence, NADH, FAD, SLS, HgCl2, fluorescence imaging|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.