Title: Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence
Authors: BEDNARKIEWICZ ArturRODRIGUES RobimWHELAN Maurice
Citation: TOXICOLOGY IN VITRO vol. 25 no. 8 p. 2088–2094
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2011
JRC N°: JRC61420
ISSN: 0887-2333
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC61420
DOI: 10.1016/j.tiv.2011.09.008
Type: Articles in periodicals and books
Abstract: A quantitative, non-destructive cellular autofluorescence based in vitro imaging assay has been developed and applied to study the cytotoxicity of Sodium Lauryl Sulfate (SLS) and HgCl2 chemical compounds on Balb 3T3 cell culture. A phenomenological double logistic model was proposed to quantify and relate the observed kinetic changes of fluorescence to the toxic potency of chemical compounds. This work forms the basis to use cellular autofluorescence measurements in the in vitro toxicity screening assays. Keywords autofluorescence, NADH, FAD, SLS, HgCl2, fluorescence imaging
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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