Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles

Purpose. To study the cytogenetic effect of exposing human peripheral blood lymphocytes (PBL) to a mixed beam of alpha particles and X-rays. Methods. Whole blood collected from one donor was exposed to different doses of alpha particles (241Am), X-rays and a combination of both. All exposures were carried out at 37 °C. 3 independent experiments were performed. Micronuclei in binucleated PBL were scored as the endpoint. Moreover, the size of micronuclei was measured. Results. Exposure of PBL to a mixed beam of high and low LET radiation led to significantly higher than expected frequencies of MN. The size measurement of MN did not reveal any differences between the effect of alpha particles and mixed beam. Conclusions. Combined exposure of PBL to alpha particles and X-rays leads to a synergistic effect as measured by the frequency of MN. From the analysis of MN distributions we conclude that the increase was due to an impaired repair of X-ray induced DNA damage.

STAAF Elina;  BREHWENS Karl;  HAGHDOOST Siamak;  NIEVAART Sander;  PACHNEROVA-BRABCOVA Katerina;  CZUB Joanna;  BRAZIEWICZ Janusz;  WOJCIK Andrzej; 

2012-11-28

SPRINGER

JRC62460

0301-634X,   

http://www.scopus.com/record/display.url?eid=2-s2.0-84864472262&origin=resultslist&sort=plf-f&src=s&st1=Micronuclei,    in,    human,    peripheral,    blood&sid=Q0b7QI3eaF2n6GqGsQ6tZJ8%3a20&sot=q&sdt=b&sl=57&s=TITLE-ABS-KEY-AUTH%28Micronuclei,    in,    human,    peripheral,    blood%2,    https://publications.jrc.ec.europa.eu/repository/handle/JRC62460,   

10.1007/s00411-012-0417-x,