This report describes the preparation, characterisation, stability and suitability studies of the certified reference material (CRM) ERM®-AD425 which contains a plasmid (pIRMM-0073) carrying a defined desoxyribonucleic acid (DNA) fragment specific for a genetic modification present in Glycine max (L.) Merill line 356043 as well as defined DNA fragment specific for the Glycine max taxon.
The CRM is available in plastic tubes containing 500µL of the plasmid DNA in 1 mmol/L Tris, 0.01 mmol/L EDTA pH 8.0 buffer (T1E0.01) and 1 ng/µL ColE1 plasmid used as background DNA. The certified values are the numbers of cloned DNA fragments for the 356043 and Ie1 PCR targets per plasmid.
CORBISIER Philippe;
BROEDERS Sylvia;
MEEUS Nele;
MAZOUA Stephane;
TRAPMANN Stefanie;
EMONS Hendrik;
2011-02-23
Publications Office of the European Union
JRC62683
978-92-79-18993-7,
1018-5593,
EUR 24687 EN,
OP LA-NA-24687-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC62683,
10.2787/36019,
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