Title: Certification of Plasmid DNA Containing 356043 Soybean DNA Fragments - Certified Reference Materials ERM®-AD425
Authors: CORBISIER PhilippeBROEDERS SylviaMEEUS NeleMAZOUA StephaneTRAPMANN StefanieEMONS Hendrik
Publisher: Publications Office of the European Union
Publication Year: 2011
JRC N°: JRC62683
ISBN: 978-92-79-18993-7
ISSN: 1018-5593
Other Identifiers: EUR 24687 EN
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC62683
DOI: 10.2787/36019
Type: EUR - Scientific and Technical Research Reports
Abstract: This report describes the preparation, characterisation, stability and suitability studies of the certified reference material (CRM) ERM®-AD425 which contains a plasmid (pIRMM-0073) carrying a defined desoxyribonucleic acid (DNA) fragment specific for a genetic modification present in Glycine max (L.) Merill line 356043 as well as defined DNA fragment specific for the Glycine max taxon. The CRM is available in plastic tubes containing 500µL of the plasmid DNA in 1 mmol/L Tris, 0.01 mmol/L EDTA pH 8.0 buffer (T1E0.01) and 1 ng/µL ColE1 plasmid used as background DNA. The certified values are the numbers of cloned DNA fragments for the 356043 and Ie1 PCR targets per plasmid.
JRC Directorate:Health, Consumers and Reference Materials

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