Verification of the Performance of MIR604 and GA21 Maize Event-specific Methods on the Maize Event MIR604 x GA21 Using Real-time PCR. Validation Report and Protocols
The European Union Reference Laboratory for GM Food and Feed (EURL-GMFF), established by
Regulation (EC) No 1829/2003, has carried out an in-house verification study to assess the
performance of two quantitative event-specific methods on maize event MIR604 x GA21
(unique identifier SYN-IR6Ø4-5 x MON-ØØØ21-9) which combines the MIR604 and GA21
transformation events. The two methods have been validated individually on single-trait events,
to detect and quantify each event in maize samples. This study was conducted according to
internationally accepted guidelines (1, 2).
In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified
food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the
implementation of Regulation (EC) No 1829/2003, Syngenta Seeds S.A.S. provided the
detection methods and the control samples: genomic DNA extracted from homogenised seeds
of MIR604 x GA21 maize (NP2171MIR604/NP2673GA21), genomic DNA extracted from
homogenised seeds of non-GM maize (NP2171/NP2673) and flour ground from seed of
NP2171MIR604/NP2673GA21 and from seed of NP2171/NP2673. The EURL-GMFF prepared the
in-house verification samples (calibration samples and blind samples at different GM
percentages).
The results of the in-house verification study were evaluated with reference to the European
network of GMO Laboratories (ENGL) method performance requirements (http://gmocrl.
jrc.ec.europa.eu/guidancedocs.htm) and to the validation results on the individual parental
events (http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm).
The results of this EURL-GMFF in-house verification studt are made publicly available at
http://gmo-crl.jrc.ec.europa.eu/.
CHARLES DELOBEL Chrystele;
PRICE Steven;
MAZZARA Marco;
VAN DEN EEDE Guy;
2011-01-28
Publications Office of the European Union
JRC62743
978-92-79-19080-3,
1018-5593,
EUR 24704 EN,
OP LB-NA-24704-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC62743,
10.2788/2878,
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