Title: Certification of a Soya 356043 Reference Material for its DNA Copy Number Ratio - Certified Reference Materials ERM®-BF425c
Publisher: Publications Office of the European Union
Publication Year: 2011
JRC N°: JRC62819
ISBN: 978-92-79-19070-4
ISSN: 1018-5593
Other Identifiers: EUR 24695 EN
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC62819
DOI: 10.2787/37327
Type: EUR - Scientific and Technical Research Reports
Abstract: ERM-BF425c is part of a set of soya CRMs containing different mass fractions of GM soya 356043. The CRM was processed and originally certified for its soya 356043 mass fraction by the European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, BE. European Commission Recommendation (EC) No 787/2004 advises to express the content of GM food and feed products as the percentage of GM DNA copy numbers in relation to target taxon-specific DNA copy numbers calculated in terms of haploid genomes, further referred to as the DNA copy number ratio. An interlaboratory comparison was conducted in 2008 to certify the existing CRM for its DNA copy number ratio. The CRM is intended for quality control of measurements of DNA copy number ratios of soya event 356043 in GM food and feed. Hereby, the plasmid DNA (pDNA) ERM®-AD425 should be used for calibration together with the soya 356043 event-specific real-time Polymerase Chain Reaction method validated by the European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) (available via: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm). ERM-AD425 contains a 99 bp fragment of the 5' plant/insert junction specific for the soya event 356043. Additionally, the plasmid carries a 243 bp fragment of the species-specific lectin gene le1, specific for the soya taxon. The CRM ERM-BF425c is available in glass bottles containing 1 g of soya seed powder, closed under argon atmosphere and is certified for its DNA copy number ratio. The minimum amount of sample to be used is 200 mg.
JRC Directorate:Health, Consumers and Reference Materials

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