Title: DNA-damage induction by eight metal compounds in TK6 human lymphoblastoid cells: Results obtained with the alkaline Comet assay
Authors: GUILLAMET EMMACREUS AMADEUFARINA MASSIMOSABBIONI E.FORTANER TORRENT SALVADORMARCOS R.
Citation: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS vol. 654 p. 22-28
Publisher: ELSEVIER SCIENCE BV
Publication Year: 2008
JRC N°: JRC63876
ISSN: 1383-5718
URI: https://www.sciencedirect.com/science/article/pii/S138357180800123X?via%3Dihub
http://publications.jrc.ec.europa.eu/repository/handle/JRC63876
DOI: 10.1016/j.mrgentox.2008.04.005
Type: Articles in periodicals and books
Abstract: Metal compounds are long-lived and can react with different macromolecules, producing a wide range of biological effects, includingDNA damage. Since their reactivity is associated with their chemical structure, it is important to obtain information on more than one compound from the same metal. In this study, the DNA-damaging potential of two mercury compounds (mercury chloride and methyl mercury chloride), two nickel compounds (nickel chloride and potassium hexafluoronickelate), two palladium compounds (ammonium tetrachloropalladate and ammonium hexachloropalladate), and two tellurium compounds (sodium tellurite and sodium tellurate) was evaluated in human lymphoblastoid TK6 cells by use of the alkaline version of the Comet assay. As the use of computerized image-analysis systems to collect comet data has increased, the metric used for quantifying DNA damage was the Olive tail moment. Treatments lasted for 3 h and the range of concentrations tested was different for each metal compound, depending on its toxicity. Both mercury agents produced DNA damage in TK6 cells, with mercury chloride producing considerably more DNA damage than methyl mercury chloride. Of the two nickel compounds, only nickel chloride (a Ni(II) compound) induced DNA breaks. Similarly, of the two palladium compounds, only the Pd(II) compound (ammonium tetrachloropalladate) was positive in the assay. Sodium tellurite was clearly positive, producing concentration-related increases in DNA damage, while sodium tellurate gave a negative response. In conclusion, the ability of inducing DNA damage by the selected metal compounds in human TK6 cells, when measured with the Comet assay, was dependent on the chemical form and, in general, compounds containing the metal in the lower valence state displayed the greater DNA-damaging ability
JRC Directorate:Health, Consumers and Reference Materials

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