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dc.contributor.authorGUILLAMET EMMAen_GB
dc.contributor.authorCREUS AMADEUen_GB
dc.contributor.authorFARINA MASSIMOen_GB
dc.contributor.authorSABBIONI E.en_GB
dc.contributor.authorFORTANER TORRENT SALVADORen_GB
dc.contributor.authorMARCOS R.en_GB
dc.date.accessioned2018-03-16T01:10:07Z-
dc.date.available2018-03-14en_GB
dc.date.available2018-03-16T01:10:07Z-
dc.date.created2018-03-14en_GB
dc.date.issued2008en_GB
dc.date.submitted2011-02-23en_GB
dc.identifier.citationMUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS vol. 654 p. 22-28en_GB
dc.identifier.issn1383-5718en_GB
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S138357180800123X?via%3Dihuben_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC63876-
dc.description.abstractMetal compounds are long-lived and can react with different macromolecules, producing a wide range of biological effects, includingDNA damage. Since their reactivity is associated with their chemical structure, it is important to obtain information on more than one compound from the same metal. In this study, the DNA-damaging potential of two mercury compounds (mercury chloride and methyl mercury chloride), two nickel compounds (nickel chloride and potassium hexafluoronickelate), two palladium compounds (ammonium tetrachloropalladate and ammonium hexachloropalladate), and two tellurium compounds (sodium tellurite and sodium tellurate) was evaluated in human lymphoblastoid TK6 cells by use of the alkaline version of the Comet assay. As the use of computerized image-analysis systems to collect comet data has increased, the metric used for quantifying DNA damage was the Olive tail moment. Treatments lasted for 3 h and the range of concentrations tested was different for each metal compound, depending on its toxicity. Both mercury agents produced DNA damage in TK6 cells, with mercury chloride producing considerably more DNA damage than methyl mercury chloride. Of the two nickel compounds, only nickel chloride (a Ni(II) compound) induced DNA breaks. Similarly, of the two palladium compounds, only the Pd(II) compound (ammonium tetrachloropalladate) was positive in the assay. Sodium tellurite was clearly positive, producing concentration-related increases in DNA damage, while sodium tellurate gave a negative response. In conclusion, the ability of inducing DNA damage by the selected metal compounds in human TK6 cells, when measured with the Comet assay, was dependent on the chemical form and, in general, compounds containing the metal in the lower valence state displayed the greater DNA-damaging abilityen_GB
dc.description.sponsorshipJRC.F.1-Health in Societyen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherELSEVIER SCIENCE BVen_GB
dc.relation.ispartofseriesJRC63876en_GB
dc.titleDNA-damage induction by eight metal compounds in TK6 human lymphoblastoid cells: Results obtained with the alkaline Comet assayen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.mrgentox.2008.04.005en_GB
JRC Directorate:Health, Consumers and Reference Materials

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