SYBR Green qPCR methods for detection of endogenous reference genes in commodity crops: a step ahead in combinatory screening of genetically modified crops in food and feed products
Identification of crops present in food and/or
feed matrices represents an important step in the screening
strategies targeting genetically modified organisms
(GMO). Soybean, maize, oilseed rape, rice, cotton, sugar
beet and potato are to date the most important sources of
genetically modified materials imported in the European
Union (EU). In order to allow detection of their presence in
an integrated screening approach, a set of SYBRGreen
real-time polymerase chain reaction (qPCR) methods has
been developed which can be used under the same assay
conditions and at similar efficiency for each of the
abovementioned crops. Each qPCR method is shown to
meet the performance criteria (i.e. specificity, limit of
detection and PCR efficiency) set by the European Network
of GMO Laboratories (ENGL). When combined with
the equivalent qPCR methods targeting GMO elements,
these crop-specific SYBRGreen qPCR methods can aid
the development of an efficient tool for determining GMO
presence in food and/or feed products.
MBONGOLO MBELLA Guillaume;
LIEVENS Antoon;
BARBAU-PIEDNOIR Elodie;
SNEYERS Myriam;
LEUNDA-CASI Amaya;
ROOSENS Nancy;
VAN DEN BULCKE Marc;
2011-11-04
SPRINGER
JRC65448
1438-2377,
https://publications.jrc.ec.europa.eu/repository/handle/JRC65448,
10.1007/s00217-010-1408-2,
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