Title: Applicability of the Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection in highly processed food matrices
Citation: EUROPEAN FOOD RESEARCH AND TECHNOLOGY vol. 234 no. 1 p. 109-118
Publisher: SPRINGER
Publication Year: 2012
JRC N°: JRC65530
ISSN: 1438-2377
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC65530
DOI: 10.1007/s00217-011-1615-5
Type: Articles in periodicals and books
Abstract: Simple tools to detect the presence of genetically modified organisms (GMO) in commercial products represent a valuable aid in managing the legal requirements for GMO testing in a cost-effective way. The 'Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection' was developed to meet such requirements and was here further tested for its applicability on detecting GMO in recalcitrant matrices. Sixty-four highly processed maize products were purchased from the market of the European Union and analysed for their GMO content. Seventy-five percent of the test samples were positive for the presence of GMO. In one sample, trace amounts of a so-called asynchronously authorized GMO could be detected. The overall outcome of the analyses indicated that a good representation of the commercially available GMO for food use could be obtained by analysing a small subset of products. Finally, the use of the ‘Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection’ in detecting GMO reliably at the 0.1% mass level is documented. The implications of these results on the further development of such type of PCR-based GMO detection systems are discussed.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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