Applicability of the Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection in highly processed food matrices

Simple tools to detect the presence of genetically modified organisms (GMO) in commercial products represent a valuable aid in managing the legal requirements for GMO testing in a cost-effective way. The 'Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection' was developed to meet such requirements and was here further tested for its applicability on detecting GMO in recalcitrant matrices. Sixty-four highly processed maize products were purchased from the market of the European Union and analysed for their GMO content. Seventy-five percent of the test samples were positive for the presence of GMO. In one sample, trace amounts of a so-called asynchronously authorized GMO could be detected. The overall outcome of the analyses indicated that a good representation of the commercially available GMO for food use could be obtained by analysing a small subset of products. Finally, the use of the ‘Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection’ in detecting GMO reliably at the 0.1% mass level is documented. The implications of these results on the further development of such type of PCR-based GMO detection systems are discussed.

KLÜGA Linda;  FOLLONI Silvia;  VAN DEN BULCKE Marc;  VAN DEN EEDE Guy;  QUERCI Maddalena; 

2012-01-13

SPRINGER

JRC65530

1438-2377,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC65530,   

10.1007/s00217-011-1615-5,