Title: Recommended protocol for the Syrian hamster embryo (SHE) Cell Transformation Assay
Authors: MAIRE MARIE-ALINEPANT KAMALAPHRAKONKHAM PASCALPOTH ALBRECHTSCHWIND KARL-RAINERRAST CLAUDINEBRUCE SHANNON WILSONSLY JAMIE E.BOHNENBERGER SUSANNEKUNKELMANN THORSTENSCHULZ MARKUSVASSEUR PAULE
Citation: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS vol. 744 p. 76-81
Publisher: ELSEVIER SCIENCE BV
Publication Year: 2012
JRC N°: JRC66211
ISSN: 1383-5718
URI: https://www.sciencedirect.com/science/article/pii/S1383571811003639?via%3Dihub
http://publications.jrc.ec.europa.eu/repository/handle/JRC66211
DOI: 10.1016/j.mrgentox.2011.12.010
Type: Articles in periodicals and books
Abstract: The Syrian hamster embryo (SHE) cell transformation assay (CTA) is a short-term in vitro assay recommended as an alternative method for testing the carcinogenic potential of chemicals. SHE cells are “normal” cells since they are diploid, genetically stable, non-tumorigenic, and have metabolic capabilities for the activation of some classes of carcinogens. The CTA, first developed in the 60’s by Berwald and Sachs (1963, 1965), is based on the change of the phenotypic feature of cell colonies expressing the first steps of the conversion of normal to neoplastic-like cells with oncogenic properties. Pienta (1977) developed a protocol using cryopreserved cells to enhance the robustness of the assay. Several variants of the assay are currently in use, which mainly differ by the pH at which the assay is performed. We present here the common version of the SHE pH 6.7 CTA and SHE pH 7.0 CTA protocols used in the ECVAM (European Centre for the Validation of Alternative Methods) prevalidation study on CTA reported in this issue (Pant et al. and Maire et al. for the SHE pH 6.7 and SHE pH 7.0 CTAs, respectively). It is recommended that this protocol, in combination with the photo catalogues presented in this issue (Bohnenberger et al. and Maire et al. for the SHE pH 6.7 and SHE pH 7.0 CTAs, respectively), should be used in the future and serve as a basis for the development of the OECD test guideline.
JRC Directorate:Health, Consumers and Reference Materials

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