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|Title:||Recommended protocol for the BALB/c 3T3 Cell Transformation Assay|
|Authors:||SASAKI KIYOSHI; BOHNENBERGER SUSANNE; HAYASHI KUMIKO; KUNKELMANN THORSTEN; MURAMATSU DAI; PHRAKONKHAM PASCAL; POTH ALBRECHT; SAKAI AYAKO; SALOVAARA SUSAN; TANAKA NORIHO; THOMAS CLAIRE; UMEDA MAKOTO|
|Citation:||MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS vol. 744 p. 30-35|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in periodicals and books|
|Abstract:||The present protocol has been developed for the BALB/c 3T3 Cell Transformation Assay (CTA), following the prevalidation study coordinated by the European Centre for the Validation of Alternative Methods (ECVAM) and reported in this issue (Tanaka et al.). Based upon the experience gained from this effort and as suggested by the Validation Management Team (VMT), some acceptance and assessment criteria have been refined compared to those used during the prevalidation study. The present protocol thus describes cell culture maintenance, the dose-range finding (DRF) experiment and the transformation assay, including cytotoxicity and morphological transformation evaluation. Use of this protocol and of the associated photo catalogue included in this issue (Sasaki et al.) is recommended for the future conduct of the BALB/c 3T3 CTA.|
|JRC Directorate:||Health, Consumers and Reference Materials|
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