Human Skin Penetration of Cobalt Nanoparticles Through Intact and Damaged Skin

Cobalt nanoparticles (CoNPs) are produced for many applications but there is a lack of data on human absorption. The aim of our study was to evaluate the CoNPs skin absorption. Experiments were performed using Franz cells with human skin. Physiological solution was used as receiving phase and 1.0 mg cm-2 of CoNPs was applied as donor phase for 24 h. Mean Co content of 8.3 ± 1.5 ng cm-2 and 1.87 ± 0.86 ug cm-2 were found in the receiving solutions of cells where the CoNPs suspension was applied on intact skin and on damaged skin respectively. Twenty-four hours Co flux permeation was 76 ± 49 ng cm-2 h-1 in damaged skin with a lag time of 2.8 ± 2.1 hours. Transmission Electron Microscope analysis on skin samples and spectroscopic analysis demonstrated the presence of CoNPs into epidermis. This study showed that CoNPs are able to penetrate the human skin in an in-vitro diffusion cell system.

LARESE FILON Francesca;  CROSERA Matteo;  TIMEUS Elena;  ADAMI Gianpiero;  BOVENZI Massimo;  PONTI Jessica;  MAINA Giovanni; 

2012-12-19

PERGAMON-ELSEVIER SCIENCE LTD

JRC66566

0887-2333,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC66566,   

10.1016/j.tiv.2012.09.007,