We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD) we measure a decrease of 6°C in the thermal unfolding of human serum albumin upon interaction with silver nano particles while this does not happen with gold. The use of SRCD allows measuring critical parameters
on protein-NP interactions and it will provide experimental data on the relative stability of key biological proteins for nanotoxicology.
LAERA Stefania;
CECCONE Giacomo;
ROSSI Francois;
GILLILAND Douglas;
HUSSAIN Rohanah;
SILIGARDI Giuliano;
CALZOLAI Luigi;
2014-01-14
AMER CHEMICAL SOC
JRC66754
1530-6984,
https://publications.jrc.ec.europa.eu/repository/handle/JRC66754,
10.1021/nl202909s,
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