Selection of test methods to be included in a testing strategy to predict acute oral toxicity: An approach based on statistical analysis of data collected in phase 1 of the ACuteTox project

More than 50 different in vitro and in silico methods assessing specific organ- and system-toxicity, such as haemato-, neuro-, nephro- and hepatotoxic ity, as well as intestinal absorption, distribution and metabolism, have been used in the firstphase of the ACuteTox project to test acommon set of 57 chemicals.This paper describes the methods used for statistical evaluation of concentration–response data collected for each of the endpoint assays, and for the development of atesting strategy applicable for acute toxicity classification of chemicals based on the achieved results of the concentration–response analysis. A final list of in vitro test methods consider ed to be promising candidates for building blocks of the testing strategy is presented. Only these selected test methods were further investigated in the prevalidation phase of the project. The test methods were chosen according to their reproducibility and reliability and most importantly, according to their potential to classify chemicals into the officialacute oral toxicity categories of the EU Classification, Labelling and Packaging (CLP)Regulation .The potential of the test methods to correctly classify the chemicals was assessed by Classificationand Regression Trees (CART)analysis.

KINSNER-OVASKAINEN Agnieszka;  PRIETO PERAITA Maria Del Pilar;  STANZEL Sven;  KOPP-SCHNEIDER Annette; 

2013-12-10

PERGAMON-ELSEVIER SCIENCE LTD

JRC66910

0887-2333,   

http://www.sciencedirect.com/science/article/pii/S0887233312003219,    https://publications.jrc.ec.europa.eu/repository/handle/JRC66910,   

10.1016/j.tiv.2012.11.010,