Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake

An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS-1 was used to evaluate the effects of silicon dioxide nanoparticles (SiNPs) exposure, in the presence or absence of macrophage-like cells THP-1. The measurements of different parameters indicated that macrophages (THP-1 cell line) can play an important role in the mechanisms of alveolar barrier damage induced by the exposure to SiNPs. It was observed a high release of TNF-α and IL-8 that could subsequently lead to down regulation of surfactant proteins A mRNA expression. The oxidative stress also contributed to the mechanism of toxicity induced by SiNPs as high reactive oxygen species production was observed. The results suggested that in vitro approach can be used to study pulmonary toxicity as long as the applied in vitro model mimics closely the complexity of vivo situation.

FARCAL Romeo;  UBOLDI Chiara;  MEHN Dora;  GIUDETTI Guido;  NATIVO Paola;  PONTI Jessica;  GILLILAND Douglas;  ROSSI Francois;  PRICE Anna; 

2013-11-18

INFORMA HEALTHCARE

JRC67299

1743-5390,   

http://informahealthcare.com/doi/abs/10.3109/17435390.2012.710658,    https://publications.jrc.ec.europa.eu/repository/handle/JRC67299,   

10.3109/17435390.2012.710658,