The Certification of Different Mass Fractions of DAS-68416-4 in Soya Seed Powder: ERM®-BF432a, ERM®-BF432b, ERM®-BF432c and ERM®-BF432d
This report describes the production of a set of Certified Reference Materials (CRMs) ERM-BF432a, b, c and d, matrix materials certified for their DAS-68416-4 mass fractions. The material has been produced following ISO Guide 34:2009 [1].Genetically modified (GM) seeds of the soya event DAS-68416-4 and of a non-GM soya variety were ground to obtain GM and non-GM base powders. A non-GM pure material was prepared and gravimetric mixtures of non-GM and GM soya powder were prepared by dry-mixing. Between unit-heterogeneity has been quantified and stability during dispatch and storage have been assessed in accordance with ISO Guide 35:2006 [2].The certified value was obtained from the gravimetric preparations, taking into account the purity of the base materials and their water mass fraction. The certified values were confirmed by event-specific real-time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025).Uncertainties of the certified values were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM) [3] and include uncertainties related to possible heterogeneity and instability and to characterisation.
The materials are intended for the calibration or quality control of methods. As any reference material, they can also be used for control charts or validation studies. The CRMs are available in glass vials containing 1 g of dried soya seed powder closed under argon atmosphere. The minimum amount of sample to be used is 500 mg.The CRM has been accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.
TRAPMANN Stefanie;
CORBISIER Philippe;
KORTEKAAS Anna Maria;
MAZOUA Stephane;
SEGHERS John;
SIMONET Julien;
2012-07-02
Publications Office of the European Union
JRC67827
978-92-79-22325-9,
1831-9424,
EUR 25105 EN,
OP LA-NA-25105-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC67827,
10.2787/55648,
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